CST Epigenetics

Understanding the role epigenetics plays in cancer initiation and progression is critical when looking for new methods of prevention, detection, and treatment. We’ve put together a starter’s guide on important epigenetic biomarkers, regulators, and histone modifications for several types of cancer.

Check out the links below to get started.

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Prostate Cancer

Androgen receptor (AR) signaling is the central signal transduction pathway in prostate cancer. Many different epigenetic mechanisms affecting this critical pathway play a role in disease initiation and progression. This includes the presence of AR variants that are constitutively transcriptionally active, interference with histone demethylase coactivators, and the formation of gene fusions of AR-responsive genes with transcription factors like ERG.

 

  1. Androgen Receptor (AR) transcripts can include cryptic exons, which result in early termination of translation and proteins lacking in the ligand binding domain. AR-V7, a variant arising from a cryptic exon, leads to aberrant transcriptional regulation and is frequently expressed in castration-resistant prostate cancer (CRPC).

    – Androgen Receptor (D6F11) XP® Rabbit mAb #5153 – WB, IP, IF, F, IHC, ChIP

Figure 1
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Androgen Receptor (D6F11) XP® Rabbit mAb.

 

– Androgen Receptor (AR-V7 Specific) (E3O8L) Rabbit mAb #19672 – WB
– Androgen Receptor Antibody (Carboxy-terminal Antigen) #54653 – WB, IP

 

  1. In some prostate cancers, Glucocorticoid Receptor (GR) signaling may aberrantly override expression of AR targets, inducing further cancer progression and AR-treatment resistance. In these instances, inhibition of Ezh2, which can act as a coactivator for Androgen Receptor, offers additional treatment options.

    – Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 – WB, IP, IF, F, IHC, ChIP, ChIP-seq

Figure 2
Chromatin immunoprecipitations were performed with cross-linked chromatin from A549 cells cultured in media with 5% charcoal-stripped FBS for 3 d and then treated with 100 nM dexamethasone for 1 hr and Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared from 5 ng enriched ChIP DNA using SimpleChIP® ChIP-Seq DNA Library Prep Kit for Illumina® #56795, and sequenced on the Illumina NextSeq. The figure shows binding across SLC19A2, a known target gene of GR (see additional figure containing ChIP-qPCR data).

 
  1. – Ezh2 (D2C9) XP® Rabbit mAb #5246 – W, IP, IF, F, IHC, ChIP, ChIP-seq

 

  1. HOXB13 is a transcription factor and marker of prostate cancer that is used to identify metastasis.

    – HOXB13 (D7N8O) Rabbit mAb #90944 – W, IP, IHC

 

  1. ERG overexpression occurs due to gene fusion with the androgen-driven promoter of the TMPRSS2 gene and is a key driver of prostate cancer metastasis.

    – ERG (A7L1G) Rabbit mAb #97249 – W, IP, IF, F, IHC

Figure 3
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma (two different cases) using ERG (A7L1G) Rabbit mAb.