The best way to ensure that your ChIP results are reliable is to use multiple validation techniques. Watch this video and learn about the different controls and experimental approaches CST scientists use to validate the antibodies recommended for ChIP-qPCR and ChIP-seq. Using validated antibodies and different verification techniques allows you to put your best foot forward.
When planning a ChIP-qPCR or ChIP-seq experiment, it is important to know when to use sonication or enzymatic methods to break up your DNA. Let our CST scientists with years of experience in developing, optimizing, and validating ChIP protocols be your guide. By clicking the below link, you can learn which type of approach and kit is right for your experiments.
Chromatin IP experiments can be a complex undertaking that require 2-3 days’ worth of effort. The CST “Guide to Successful Chromatin IP and Chromatin IP Sequencing” highlights the critical steps in ChIP protocols and provides the supporting data to explain the recommendations we make for CST antibodies, controls, and ChIP reagents.
In this app note, we walk you through how to create the most optimal DNA library for your experiments. Important considerations that are discussed include how much ChIP-DNA is needed, how to pool DNA libraries, and how to check the quality of the DNA library that you have before performing in-depth sequencing analyses.
CST offers a variety of validated ChIP kits, reagents, and tools for your ChIP-qPCR or ChIP-seq experiments. On this resource page, you can find the additional products you need to complete your experiment, whether that may be a whole kit, buffers, beads, or even a magnetic separation rack.
CST offers an extensive selection of antibodies validated for use in ChIP-pPCR and ChIP-seq applications. On this resource page, you can find ChIP or ChIP-seq-approved antibodies against a wide variety of targets. On the product page of each antibody, you can then find validation testing data.
Using the most appropriate positive and negative controls in your ChIP experiments is extremely important to properly verify your results. To find the best primer pairs that work with each one of our validated ChIP antibodies in the designated cell model (shown in the ChIP data legend on the antibody product page), check out this resource page.
Successful ChIP experiments require antibodies that are sensitive, specific, and reliable. In order to provide the best reagents for our customers, CST has a uniquely rigorous approach to validating the antibodies we recommend for ChIP-qPCR and ChIP-seq. Hear why Dr. Kadoch appreciates our thorough validation process performed in-house.
Chromatin IP experiments can be time-consuming and technically challenging. Before diving into your next experiment, check out answers to some of the most common ChIP protocol questions, so that you can plan ahead with the help of our expertise.
As experimental issues arise, let our expertise help you to resolve them with this troubleshooting guide that provides concrete steps and protocol tweaks that CST ChIP experts recommend for various experimental issues, such as low chromatin yield, and sub-optimal chromatin fragmentation.